VION FAST LIQUID CHROMATOGRAPHY SYSTEM
The Vion Liquid Chromatography System offers quaternary solvent blending and advanced separation performance. It is specifically designed for routine analysis, research and method development, making it ideal for fast liquid chromatography applications that demand high resolution and sensitivity.

The Vion Liquid Chromatography System offers quaternary solvent blending and advanced separation performance. It is specifically designed for routine analysis, research and method development, making it ideal for fast liquid chromatography applications that demand high resolution and sensitivity. The system features a quaternary pump with an online degasser, a precise injector, and is compatible with various detectors, including UV, PDA, fluorescence (FLR), refractive index (RI), and evaporative light scattering detectors (ELSD). It also supports columns with particle sizes as small as 1.7 microns and can handle high-pressure operations. Additionally, the Vion system is fully compatible with mass spectrometers, delivering accurate and reliable results for complex analytical workflows.
| Parameter | Specification |
|---|---|
| Quantum synchronization | Injection synchronization between pump and sample manager enhances retention time reproducibility |
| Flow rate range | 0.001 to 2.000 ml/min with increment of 0.0001 ml/min |
| pH range | 1 to 13 |
| Cycle time | ≤20 s inject-to-inject |
| Maximum operating pressure | 18,000 psi |
| Leak sensors | Available |
| Dwell volume (total system) | ≤350 µL |
| Gradient delay volume | ≤310 µL |
| Solvent configuration | Four-solvent low-pressure gradient |
| Gradient type | Quaternary gradient, Low-pressure mixing |
| Gradient profiles | 12 gradient curves |
| Pressure pulse | ≤1.0% |
| Number of solvents | 4 |
| Solvent degassing | Automated vacuum degassing |
| Solvent mixing | Automated |
| Pump flow rate range | 0.01 to 2.0 ml/min |
| Flow precision | ≤0.070% RSD |
| Flow accuracy | ±1.0% |
| Composition precision | ≤0.10% RSD |
| Composition accuracy | ±0.25% |
| Pump seal wash | Automated Seal wash |
| Automated priming | Yes |
| Sample plates | Any two of the following: 96 and 384 microtiter plates, 48 position 2.00-ml vial plates |
| Injection method | Total-volume sample injection |
| Injection precision | ≤1% RSD |
| Injection accuracy | ±0.1 µL |
| Injection linearity | ≥0.9999 |
| Injection volume range | 0.1 to 100.0 µL |
| Advanced sample manager | Auto-dilution and Auto-addition |
| Injection needle wash | Integral, active, programmable |
| Sample compartment temperature | 4.0 to 45.0 °C, settable in 0.1 °C increments |
| Sample temperature accuracy | ±0.5 °C at sensor |
| Sample temperature stability | ±1.0 °C at sensor |
| Sample carryover | ≤0.002% caffeine (UV) |
| Column capacity | Two columns |
| Column heating/cooling | Forced air circulation method |
| Solvent conditioning | Active pre-heating |
| Column tracking | Yes |
| Column compartment temperature stability | ±0.2°C |
| Column compartment temperature range | 4.0 to 110.0°C, settable in 0.1°C increments |
| Column compartment temperature accuracy | ±0.5°C |
| Sample organizer temperature accuracy | ±1.0 °C at the sensor |
| Sample organizer temperature stability | ±1.0 °C at the sensor |
| External control | Spera Analytical Software |